A new paper published in Glia by Miklós Zöldi
The paper showcases a new method enabling nanoscale molecular measurements within ultrathin astrocytic processes (~10–100 nm) with single-molecule localization microscopy.
Astrocytes, these star-shaped glial cells of the central nervous system, play essential roles in supporting the function of neurones. By using postnatal electroporation of mice, we achieved a selective and sparse labeling of the astrocytic population with fluorescent proteins delineating the plasma membrane of the astrocytic arbor.
To show the potency of the method, we studied the differential distribution of monoacylglycerol lipase (MAGL) within neuronal and astrocytic processes with dual-color STORM super-resolution imaging. MAGL regulates synaptic plasticity in neurons and couples endocannabinoid signaling to prostaglandin synthesis and neuroinflammatory processes in astrocytes.
, and . 2026. “ A Super-Resolution Approach for Astrocyte-Specific Molecular Imaging Reveals the Nanoscale Distribution of Monoacylglycerol Lipase, the Metabolic Node Between Endocannabinoid and Prostaglandin Signaling.” Glia 74, no. 9: e70186. https://doi.org/10.1002/glia.70186.
